The United States women's medley relay finished second behind Canada's foursome of Taylor Ruck, Sydney Pickrem, Maggie MacNeil and Kayla Sanchez.
The men's team didn't perform up to their standards, coming within 0.34 second of missing the 4x100m medley relay final.
Both the men's and women's 4x100m medley relay final takes place Saturday, July 31, beginning at 9:30 p.m. They will be aired on NBC.
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The U.S. has historically owned the 4x100m medley relay on the men's side, winning it at every Olympics since its debut in 1960 except for the boycotted 1980 Moscow Games. They are going for a 10th consecutive gold at the Tokyo Games.
Meanwhile, the U.S. women have been almost just as dominant, winning 10 of the event's 15 all-time golds.
Watch all the action from the Tokyo Games Live on NBC