The University of North Texas has suspended Pi Kappa Phi through 2020.
An investigation found members of the fraternity participated in hazing.
In September video revealed an incident in February where one brother hit another brother in the face.
Members of the fraternity filed an appeal, but it was denied. All members of the house must now be out by the end of the month.
Correction: An earlier version of this story misidentified the fraternity that was suspended.