The pair has reportedly broken up before (in August 2008), but sources tell People it's over.
"They had some disagreements and decided to not continue to see each other," says one source tells People.
The couple's publicists have yet to confirm the breakup.
What the El!?! Editorial:
Thank goodness, I can't stand John Mayer. I think he's using her for publicity.
Case in point, this news comes a day after news that Mayer was working with a jeweler to create an engagement ring for Aniston.
A 'pal' told The Mirror: "Money is no object for John. He is creating a vintage-style classic ring for Jen. He's looking at 18-carat flawless diamond…"
How much do you want to bet that he's the source of the story. He's such a media whore.